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Growth curve and spot assays were conducted in minimal growth medium MMB liquid or agarized12 and supplemented with the indicated compound (Sigma-Aldrich) at the desired concentration.
This inhibitory effect of rich medium can be reproduced by just adding the common amino acids to minimal growth medium (A94).
(15) UV-irradiated E. coli K12 uvrA BC) cells show higher survival if plated on minimal growth medium (MM) rather than on rich growth medium (RM).
Their experiments involved first exposing the mold to mutation-inducing X-rays and then culturing it in a minimal growth medium that contained only the basic nutrients that the wild-type, or nonmutated, strain of mold needed to survive.
In minimal growth medium, the uvrA and uvrB strains had the largest µ (1.9 - 6.2-fold greater than that for isogenic wild-type strains, depending on the mutation assay).
Experiments, in which multiple treatments of natural and artificial seawater were tested, as well as calcium-addition experiments, demonstrate that calcium inhibits complete dissolution of the minimal growth medium in wells.
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Also, analysis of gene essentiality of Y. lipolytica cells growth in minimal growth (MG) medium or yeast extract (YE) medium was conducted, and the essential genes predicted based on four Y. lipolytica models in YE medium were further verified by database of essential genes (DEG).
The verification and analysis included optimal cell growth in minimal growth (MG) medium (Lanciotti et al. 2005) and utilization of different carbon sources.
However, extracellular and intracellular compartments are specified, and a minimal extracellular growth medium was applied to all models, along with necessary transport reactions that allow for its uptake.
To determine if GNA-2-deficient embryos could polarize if provided with synthetic osmotic support, worms expressing PAR-2 GFP PAR-2 GFPcted to gna-2 RNAi, and embryos were disubjectednd incubatod in minimal embryonic growth medium (EGM) [ 55].
We seeded 100 UACC-812 breast cancer cells into individual wells of a 96-well cell culture plate containing a confluent monolayer of fibroblasts growing in minimal essential growth medium supplemented as described above.
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