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The minimal gametocyte count was chosen based on infectivity to mosquitoes [ 8].
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If sexual forms were detected, a gametocyte count was performed against 1,000 WBC.
One positive gametocyte count at any time after treatment during the follow-up period was enough to define gametocyte carriage, while a complete set of negative counts during follow-up was required to confirm no carriage.
The median (range) gametocyte count was 41/µL (16–110/µL).
20 If gametocytes were seen, a gametocyte count was performed against 1,000 leukocytes.
If P. falciparum gametocytes were seen, a gametocyte count was performed against 1,000 leukocytes.
If gametocytes were seen, the gametocyte count was extended to 1,000 WBC.
A rising gametocyte count does not indicate treatment failure.
Patients who were at least 16 years old with uncomplicated Plasmodium falciparum infection, provided written informed consent, and had a gametocyte count > 55/μl within 72 hours of diagnosis, regardless of asexual parasite counts, were eligible for enrollment.
Asexual parasite and gametocyte counts were recorded separately at screening; total parasite counts were recorded at all other assessments.
The median gametocyte counts were 0 prior to dosing and at each time point following dosing for both treatment groups.
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