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It was therefore of interest to determine the context of this 18S rRNA complementary region within the global RNA secondary structure of the minimal functional sequence, and to compare the structure with that determined for the influenza BM2 signal [19].
By using a reporter assay and Annexin-V staining, the minimal functional sequence of FHL1C inhibiting RBP-J-mediated Notch transactivation and inducing cell apoptosis was identified.
Using various truncated forms of FHL1C, we found that the RBP-J-binding motif of FHL1C had almost the same effect as full-length FHL1C on the induction of apoptosis, suggesting that the minimal functional sequence in the RBP-J-binding motif of FHL1C might be a new drug candidate for T-ALL treatment.
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These HSEs have binding affinity comparable to the minimal functional binding sequence as long as they exceed 4 subunits [20].
E. coli oriC spans the minimal functional 245-bp sequence comprising two parts, the AT-rich DNA unwinding element (DUE) and the DnaA-binding region (Bramhill & Kornberg 1988).
Subsequent modifications of the minimal promoter moiety of the Ptet promoters followed a rational design strategy, stepwise altering known functional sequence elements within the CMV minimal promoter as used in Ptet-T1.
Under scenario (ii) all species are functionally similar resulting in minimal functional complementarity and maximal functional redundancy.
In addition, to insure the inclusion of genes with minimal functional characterization, we included 10 genes represented only by expressed sequence tags (ESTs).
To identify the minimal functional unit for silencing, we further dissected the repeat A sequences.
In general, ABC permeases show the highest level of sequence similarity over the C-terminal five transmembrane regions, and this is considered to be the minimal functional unit [ 11].
We define a novel characterization of minimal functional dependencies.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com