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These genes have minimal functional annotation with scores below 3.5 and represent over 75% of genes neither protected by patents nor targeted by small molecule drugs.
Based on comparative genome analysis across multiple organisms, protein families have been identified containing domains for which minimal functional annotation is available.
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We carried out gene ontology (GO) analysis on the functional annotation of 6,327 (34.3%) genes with minimal introns (Table S1), and found several significantly overrepresented functional groups (Figure S1).
Functional annotation gave information on protein function annotation, pathway annotation, COG annotation and Gene Ontology (GO) annotation.
Functional annotation of orthologs is derived from previously annotated genomes.
Subjecting all genes expressed per sample to functional annotation using MapMan [ 27] shows that difference between functional category distributions among the samples is minimal.
The results were classified as "functional annotation clustering," "functional annotation chart" and "functional annotation table".
Functional annotation of the proteins was performed using GO and KEGG annotation.
Functional annotation of variants was performed using Polyphen-2 [22] and the Combined Annotation Dependent Depletion method (CADD) [23].
Furthermore, a database was also integrated for storing, retrieving and performing the detailed functional annotation studies.
Our association results and functional annotation suggest the involvement of new genes in human erythropoiesis.
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