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Earlier measurements of energy transfer from IsiA supercomplexes to PSI indicate very high efficiencies and minimal fluorescence emission [36].
The photosynthetic performance of light adapted plants was evaluated by monitoring the effective quantum yield of photosystem II (ΦII) defined as (FM'- F0')/ FM' [ 91], where FM'and F0' represent the maximal and minimal fluorescence emission of photosystem II under light conditions.
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NBD-cholesterol showed minimal fluorescence in aqueous buffer with an emission maximum (λmax) equal to 545 nm.
The melting temperature (Tm) for each sample was determined by quantifying the temperature at which the first derivative of fluorescence emission reached a minimal value (Protein Thermal Shift software).
Table 1 Analysis of spatial distribution of fluorescent pigments and ratio of fluorescence emission spectra.
Fluorescence emission at the tumor location, given as relative units after normalization of maximal and minimal intensities, was measured with regions of interest of equal size at specified time points.
Summary of the maximal D-CaM fluorescence emission.
Confocal fluorescence microscopy was used to quantitatively visualize the EtNBS construct via its 670 nm fluorescence emission peak.
Absorption and fluorescence emission spectra were determined.
A blue fluorescence emission was also observed in identical cells under the ThT fluorescence channel and in close proximity to those structures producing lumogallion fluorescence (Fig. 3j).
Mechanical transitions corresponding to DNA hybrid rupture occurred simultaneously with discontinuous changes in the fluorescence emission.
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