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Herein, we report a straightforward approach for minimal fluorescence background device fabrication, in which filter paper was shaped and laminated in between two biaxially oriented polypropylene (OPP) and polyvinyl butyral (PVB) composite films.
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The minimal fluorescence.
minimal fluorescence yield of a "dark-adapted" sample.
Chloroplasts are recognized by their high fluorescence background.
FI = mean fluorescence sample – mean fluorescence background)/ mean fluorescence no peptide – mean fluorescence background).
The F0 (minimal fluorescence), Fm (maximal fluorescence), Fv (variable fluorescence) and Fv/ Fm were recorded.
In our analysis, a slight red (soft pink) fluorescence was established as the minimal fluorescence signal.
Minimal background fluorescence was observed.
Owing to the extraordinarily high quenching efficiency of GO, the probe labeled with a fluorophore carboxyfluorescein exhibits minimal background fluorescence, while strong fluorescence intensity is observed with the addition of Ago2 miRNA complex due to the scission of the target mRNA sequences and the release of FAM-linked oligonucleotide fragments.
In comparison to the minimal background fluorescence of wild-type (N2) animals, the total spot number, area or fluorescence intensity per animal outputs of the SpotDetector BioApplication were markedly increased in transgenic animals (Figure 2O Q).
The strong interaction between nucleobases of the loop structure and RGO nanosheet made the MB-AgNCs-RGO system exhibit minimal background fluorescence.
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