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PGC-1α expression reached the minimal expression level at 0.1 μmol/l.
A second requirement is for minimal expression level in the normal tissue, to avoid autoimmune complication.
The concomitant minimal expression level of PRDM14/PRDM14 in our cells suggested that different regulatory mechanisms underlie human and mouse PGC specification and development (Fig 5E).
Based on our prior experience with prevention of inhibitor formation, one would predict a minimal expression level to be required for reversal.
Therefore, the minimal expression level required for inhibitor reversal is slightly higher but still similar to that required for prevention of inhibitor formation in previously untreated animals.
Those genes which displayed a minimal expression level of -2 and an up- or downregulation of at least 2-fold were selected for pathway analysis, using DAVID and KEGG provided by the National Center for Biotechnology Information (NCBI).
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In the case of a transcriptional activator, full and zero occupancy correspond to maximal and minimal expression levels, respectively.
In contrast to these findings in mouse cell lines, we found expression of both IGFBP-5 paraloguesignificantlyly decreased from 2 d, reaching minimal expression levels by 5 d for IGFBP-5.1 (Fig. 3C) and 8 d for IGFBP-5.2 (Fig. 3D).
log Cy5/Cy3 ratios were calculated after passing quality check and minimal expression levels.
The minimal expression levels of the SM22 promoter in the HEK293 cells did not achieve episomal retention of this plasmid.
Almost all the genes in glycolysis and the TCA cycle appeared to have minimal expression levels at 21 DAP.
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