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A minimal expression change of 3.0 and 0.3 fold had to be reached to be considered as an up-regulated or down-regulated candidate gene respectively.
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miR-450 showed minimal expression changes (FC = −1.04; data not shown).
cDNA generation and quantitative RT-PCR was performed as described previously [ 50] using eight control genes with minimal expression changes across all tested conditions for data normalisation.
We considered the impact of several technical variables including array batch, RNA preservative, RNA quality (RIN) and anatomic location of the lung biopsy; minimal expression changes were associated with these variables and we therefore did not include them in the final model.
A minimal expression fold change of ≥2 in all three replicates is indicated with a +.
Only genes showing a minimal expression fold change of two and a p-value ≤0.05 were considered as being differentially expressed.
As a subset of Additional file 1, this list contains all 334 transcripts, represented by respective contig numbers, with a minimal expression fold change of two between the two petal types.
All 600 genes show a minimal expression fold-change of two and a p-value equal to or below 0.05.
Array results for the LuCaP xenografts at various passages showed minimal gene expression changes during serial transplantation in mice, from p64 to p99 for LuCaP 35 and p40 to p59 for LuCaP 49.
Fold change indicates the ratio of maximal and minimal expression in summer.
There were a minimal number of global gene expression changes for the NOEL (0.02 mg/kg), mid dose (2 mg/kg) and the highest dose (20 mg/kg).
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