Sentence examples for minimal essential tissue from inspiring English sources

Microarray analysis was employed to identify global gene expression changes of EHEC O157∶H7 grown in the presence of epithelial cells, relative to the same organism grown in the absence of epithelial cells (Penassay broth, minimal essential tissue culture medium or minimal essential tissue culture medium in 5% CO2).

Down-regulation of 299 genes and up-regulation of 105 genes was reported following EHEC growth in the presence of rabbit erythrocytes, compared to culture of bacteria in minimal essential tissue culture medium alone [22].

Prior to bacterial infection, cells were incubated (24 hr at 37°C in 5% CO2) in minimal essential tissue culture medium without antibiotics and fetal bovine serum, as previously described [5].

This is the first study to characterize gene expression changes of EHEC O157∶H7 grown in the presence of epithelial cells, compared to bacteria cultured in either minimal essential tissue culture medium (in the presence and absence of 5% CO2) or Penassay broth alone.

Trypsinized cells were then pelleted, centrifuged at 500 rpm for 5 min (Beckman Coulter, Mississauga, Ontario), resuspended in minimal essential tissue culture medium, and re-seeded into either T25 flasks or 6-cm Petri dishes (Becton Dickinson Labware, Franklin Lakes, NJ) and again grown at 37°C in 5% CO2 until confluent.

Tissues were homogenized in 1 mL plain Dulbecco minimal essential medium, and tissue and blood samples were serial diluted 10-fold.

On the 5th day, EBs were collected and plated in complete α-modified Eagle's minimal essential medium in tissue culture dishes in the absence of inducers (NI) until day 14.

Using a TissueLyser (QIAGEN Inc, Valencia, CA, USA), we homogenized ≈2 10 mg of tissue in minimal essential medium (Eagle) supplemented with 20% fetal bovine serum, l-glutamine, penicillin, streptomycin, gentamicin, and fungizone.

The cell lines were kept under standard tissue culture conditions using Minimal Essential Medium containing 10% foetal calf serum (FCS).

Fibroblast cells were cultured in plastic tissue culture flasks containing minimal essential medium (MEM) supplemented with 10% FBS, L-glutamine (2 mM/L), penicillin (100 mg/ml), streptomycin (100 mg/ml) and Amino-Max™.

A tissue-independent development-related minimal essential network derived from this interactome model was associated with the same evolutionarily conserved growth pathways identified in the main data set.