Eagle's minimal essential medium.
The culture medium was a 1 1 mixture of Dulbecco/Vogt modified Eagle's minimal essential medium (DMEM) and F12 (Invitrogen, USA) supplemented with 10% fetal bovine serum (Invitrogen).
All cell lines were grown in Eagle's minimal essential medium (MEM) containing 10%% (v/v) heat-inactivated fetal bovine serum, 100 IU penicillin, and 100 μg/mL streptomycin.
Phosphate-buffered saline (PBS; 10 mM, pH 7.4), Dulbecco's modified Eagle medium (DMEM), and minimal essential medium (MEM) were purchased from Gibco (Life Technologies Corporation, Carlsbad, CA, USA).
Adherent cells were routinely cultured in Dulbecco's minimal essential medium (DMEM), and leukemia cells in RPMI 1640, in an environment of 5% CO2, 85% humidity, and 37 °C.
Minimal essential medium (MEM -alpha and the osteoblast MEM -alphaell line were purchandd from the Kosteoblastbank (Seoul, South Korea).
The cells were maintained in minimal essential medium (Sigma-Aldrich) supplemented with 10% fetal bovine serum, 1% non-essential amino acids and an antibiotic-antimycotic mixture.
PC-3M-luc-C6 cells were grown in minimal essential medium (ATCC) (For supplemental data)[30].
After centrifugation, the plates were incubated in Eagle's minimal essential medium as described above.
NIH3T3 cells were cultured in Dulbecco's modified Eagle's minimal essential medium.
P19CL6 cells were grown in Dulbecco-Modified Minimal Essential Medium supplemented with 10% fetal bovine serum.
