Sentence examples for minimal essential media from inspiring English sources

Exact(34)

Vitamins from Eagle's minimal essential media (folic acid, nicotinamide, riboflavin, cobalamine) also positively influenced growth of ureaplasmas, but were not essential [52].

Intact and disrupted CPMV-UV2.0 and CPMV-WT particles were incubated with HeLa human epithelial cells cultured in Dulbecco's minimal essential media (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% L-glutamine, and 1% penicillin/streptomycin.

Briefly, hippocampi were dissected in ice-cold dissecting medium (50% Minimal Essential Media (MEM) with no bicarbonate, 50% calcium and magnesium-free Hank's Balanced Salt Solution (HBSS), 7.5 mM D-glucose, 20 mM HEPES, pH 7.15).

For the metabolic labeling experiments, mid-logarithmic growth T. brucei 221VG1.1 cells induced with tetracycline (1 µg ml−1) for different times were centrifuged (800 g 10 minutes) and washed in minimal essential media minus either methionine or serine, before resuspension in the same media at 1×107 cells ml−1.

Skin explants were placed into 100 mm2 cell culture dishes dermal side down, covered with sterile, ethanol-washed glass coverslips and Dulbecco's Minimal Essential Media (DMEM; Gibco, Carlsbad, CA) supplemented with 10% Fetal Bovine Serum (FBS; Lonza, Walkersville, MD) and 2% antibiotic/antimycotic (Gibco, Carlsbad, CA).

These cells were cultured in Dulbecco's minimal essential media (DMEM) containing 10% fetal bovine serum (FBS).

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Similar(25)

Eagle's minimum essential media.

HEK 293 cells were cultured in alpha-minimal essential media containing 10% FBS at 37°C with 5% CO2.

Cells were maintained in α-minimal essential media (α-MEM; GIBCO) supplemented with 10% fetal bovine serum (FBS; Cansera International Inc), 0.017% penicillin G and 0.01% gentamycin in a humidified incubator at 37°C and 5% CO2.

For example, he used only one recipe for the cell culture media: minimal essential medium (MEM a liquid solution, originally invented by Harry Eagle, which meets the basic requirements for many cells.

Both cell lines were maintained at 37 °CC in a 5%% CO2 humidified incubator and their cell-specific culture media (minimal essential medium and McCoy's 5A medium, respectively), supplemented with 10%% heat-inactivated fetal bovine serum (FBS), 100 U/ml of penicillin G, and 100 μg/ml of streptomycin, which were changed every 3 days.

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