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Vitamins from Eagle's minimal essential media (folic acid, nicotinamide, riboflavin, cobalamine) also positively influenced growth of ureaplasmas, but were not essential [52].
Intact and disrupted CPMV-UV2.0 and CPMV-WT particles were incubated with HeLa human epithelial cells cultured in Dulbecco's minimal essential media (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% L-glutamine, and 1% penicillin/streptomycin.
Briefly, hippocampi were dissected in ice-cold dissecting medium (50% Minimal Essential Media (MEM) with no bicarbonate, 50% calcium and magnesium-free Hank's Balanced Salt Solution (HBSS), 7.5 mM D-glucose, 20 mM HEPES, pH 7.15).
For the metabolic labeling experiments, mid-logarithmic growth T. brucei 221VG1.1 cells induced with tetracycline (1 µg ml−1) for different times were centrifuged (800 g 10 minutes) and washed in minimal essential media minus either methionine or serine, before resuspension in the same media at 1×107 cells ml−1.
Skin explants were placed into 100 mm2 cell culture dishes dermal side down, covered with sterile, ethanol-washed glass coverslips and Dulbecco's Minimal Essential Media (DMEM; Gibco, Carlsbad, CA) supplemented with 10% Fetal Bovine Serum (FBS; Lonza, Walkersville, MD) and 2% antibiotic/antimycotic (Gibco, Carlsbad, CA).
These cells were cultured in Dulbecco's minimal essential media (DMEM) containing 10% fetal bovine serum (FBS).
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Eagle's minimum essential media.
HEK 293 cells were cultured in alpha-minimal essential media containing 10% FBS at 37°C with 5% CO2.
Cells were maintained in α-minimal essential media (α-MEM; GIBCO) supplemented with 10% fetal bovine serum (FBS; Cansera International Inc), 0.017% penicillin G and 0.01% gentamycin in a humidified incubator at 37°C and 5% CO2.
For example, he used only one recipe for the cell culture media: minimal essential medium (MEM a liquid solution, originally invented by Harry Eagle, which meets the basic requirements for many cells.
Both cell lines were maintained at 37 °CC in a 5%% CO2 humidified incubator and their cell-specific culture media (minimal essential medium and McCoy's 5A medium, respectively), supplemented with 10%% heat-inactivated fetal bovine serum (FBS), 100 U/ml of penicillin G, and 100 μg/ml of streptomycin, which were changed every 3 days.
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