RPMI-1640 and minimandessential minimalMessentialculture media, penicillin/streptoMEMin, horse serum, and GelBond film were purcelled from Lonza.
HeLa cells and human skin fibroblasts were maintained in low glucose Dulbecco's minimal essential medium (DMEM), HEK cells, HT1080, U118, U373 and COS-7 cells in high glucose DMEM.
At the time of the assay, virus-containing supernatants were thawed and immediately serially diluted in dilution media (unsupplemented Eagle's minimal essential medium for MDCK cells) prior to use in TCID50 assays.
The inactivated sera were mixed with 30 μl of virus suspension (USUV strain Vienna 2001 [ 26]), incubated at 37°C for 60 min and then supplemented with 60 μl of cell suspension (porcine kidney cells in Minimal Essential Medium with 3% fetal calf serum).
Following preliminary studies testing cell concentrations, the work reported here used cell suspensions containing an average of 100,000 cells in Minimal Essential Medium with 20% fetal bovine serum (MEM20); this concentration was injected into each sponge cube with a pipette.
Adherent cells were routinely cultured in Dulbecco's minimal essential medium (DMEM), and leukemia cells in RPMI 1640, in an environment of 5% CO2, 85% humidity, and 37 °C.
HT-29 and HCT 116 cells were maintained in McCoy's 5A medium (Invitrogen, Leiden, The Netherlands); CaCo-2 and C643 cells, in minimal essential medium (Eurobio); and MCF-7, SKBR3, MDA-MB-231, ML-1, SCC-14C, and SCC-22A cells, in Dulbecco's modified Eagle's medium (DMEM; Biochrom KG, Berlin, Germany).
T cell epitope-based vaccines provide the minimal, essential information required for protective immunity T cell epitopes are critical mediators of cellular immunity.
For example, he used only one recipe for the cell culture media: minimal essential medium (MEM a liquid solution, originally invented by Harry Eagle, which meets the basic requirements for many cells.
CO2 depletion was accomplished by inoculating tachyzoites in host cells in minimal essential medium without NaHCO3 but containing 25 mM HEPES.
