Exact(8)
In this report, we confirm that cytosolic filamentous structures formed by the PSTPIP1 protein are membrane associated and we identify the minimal domains of PSTPIP1 that are required to form these filamentous elements.
Domain mapping shows that the minimal domains required for methylation include the highly conserved SPRY domain at the Ash2L C-terminus and an acidic polypeptide of 26 amino acids from RbBP5.
In order to study the underlying mechanism for Ash2L/RbBP5 mediated stimulation of MLL1SET activity, we first mapped Ash2L and RbBP5 for minimal domains that support the overall activity of the MLL1 core complex.
Taken together it indicates that the kinase domain in Ack1 and tyrosine176 in the kinase domain along with AKT PH domain appear to be minimal domains required for efficient Ack1/AKT complex formation.
To map the interactions between TUTs and pre-miRNA, we first sought to identify the minimal domains used for pre-miRNA recognition and mono-uridylation.
Interestingly, the putative Dicers from metazoan species formed two distinct clades, one containing minimal domains and the other containing multiple additional domains (Additional files 3 and 4).
Similar(52)
We argue that previous problems with tunnelling are symptoms of overly general designs, and we demonstrate a minimal domain-crossing primitive which nevertheless achieves the majority of benefits possible from tunnelling.
PA-Av/IFA also induced PA-specific IgG; however minimal domain 4-specific IgG was detected.
We next sought to determine the minimal domain within the V3 region required for SU-CXCR4 interaction.
The activation domain of the GmGT-2B was also analyzed in detail and it appears that the N-terminal peptide of 153 residues is the minimal domain for transcriptional activation.
However, the minimal domain of RanBP2/Nup358 required for in vitro E3 activity does not include the SIM, although it is directly adjacent to it, and it has been shown to bind SUMO in vitro [22], [29].
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