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Moreover, a minimal detection limit (DL) is obtained as good as 1.24×10−5.
The minimal detection limit (MDL) in the human subjects was 28.0 μgAl/gCa, whereas it was 19.5 μgAl/gCa in calibration standards.
The minimal detection limit for total VEGF was 31 pg ml−1.
The minimal detection limit for VEGFR-2 was 78 pg ml−1.
The minimal detection limit for free VEGF was 20 pg ml−1.
The minimal detection limit for Her-2/neu was 24 pg ml−1.
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When the result was below the minimum detection limit for the assay, the minimal detectable concentration was used for the analyses.
The minimal detection limits were: for sIL-2R : 16pg/ml, for sIL-6R : 8 pg/ml and for sTNF-R1 : 0.03 ng/ml.
The minimal detection limits for the TGF-β1 and IL-17A ELISA kit were 4.61 and 4 pg/mL, respectively.
However, even with this in mind, our result in collagen type I based phantoms with NIHOVCAR3 cells indicate possibility of extremely low minimal detection limits.
The minimal detection limits determined for the novel real-time PCR described here were in the range of 1 to 10 CFU per reaction in whole blood spiked with E. coli or S. aureus, depending on DNA extraction methods; as expected, this indicates that the DNA purification step can have a profound effect on the final results.
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