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This indicates that the deployment of vertical arrays of trays with a minimal depth of 1.0 m may efficiently utilize the water column to grow out juvenile C. virginica.
Hence, we have Foreman describing Net-geners (and let them not take the rap for us all, we are all doing this!) as pancake people -- able to cover and have access to enormous ranges of information yet having minimal depth of knowledge within.
To trigger only a minimal unspecific physiological stimulus, sham acupuncture is applied with a minimal depth of needling (not exceeding 5 mm), avoiding real acupoints.
Sequencing on each the SOLiD and Genome Analyzer platforms was completed independently, with assembly algorithms each requiring a minimal depth of 3 reads in order to confirm an allele call.
Variants were called using bcftools and then filtered using vcfutils varFilter (minimal depth of 8, maximal depth of 100, Phred scores of SNP call ≥ 30, and no indel present within a 2 bp window) as previously reported [ 6].
The libraries were sequenced with paired-end (2 × 100 bp) runs using Illumina HiSeq 2500 sequencers to a minimal depth of 30× base coverage (average depth of ∼36.9× base coverage).
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If we increased the minimal depth to 8, the number of SNPs dropped to 3,173,618 but several polymorphisms previously detected by Sanger sequencing were no longer detected, in particular in the three lines with a depth of coverage lower than 10x (Levovil, Ferum and Criollo).
Assuming that five reads is a minimal depth to reliably determine the sequence of a haploid genome (K eightley et al. 2009), we see in Figure 3B that >97.8% of ycnbwsp_3-HE and 96.7% of ycnbwsp_2 are covered with five or more reads.
The minimal depth 8.99 fold covering 93.71% of the mt genome (see Table 1) suggests that there was sufficient content to detect the variation.
We generated a total of 23.15 gigabases (Gb) clean sequencing reads from four libraries of pooled intestinal samples (5Gb minimal for each library), reaching a minimal coverage depth of 12.5 per strand for each (Additional file 1: Table S2).
The samples were studied with a Leica multiphoton system using a titanium sapphire laser (excitation 800 nm with pulses in the 200 fs range) to obtain a stack of two-dimensional (2D) images to a minimal focus depth of 120 μm.
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