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The minimal cut off of 80,000 μm was determined by optimising the LMD tissue area (71,284 μm) required to obtain adequate RNA sample for successful semi-quantitative PCR analysis of Hypoxanthine Phosphoribosyltransferase 1 (HPRT1).
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For metagene analysis, we identified 'null-peak' tiled regions as those having all probe ratios below the minimal cut-off value for peak detection.
GeneIndexer generates correlation scores (0.1 minimal cut-off to 1.0) for a specific gene in the geneset to the interrogation term using a species-specific scientific abstract database containing over one million human-curated entries.
As an evaluation of the internal consistency reliability (ICR) of each construct, the values for Cronbach's alpha (CA) exceed 0.70 the minimal cut-off as recommended by Nunnally [ 83] and Nunnally et al. [ 84].
Significance of the biological-function representation in iHSLN was evaluated by means of a hypergeometric test using Benjamini & Hochberg false discovery rate with a P-value < 0.05 as a minimal cut-off.
Using a minimal cut-off of five genes per group, nine gene sets were identified; seven showed higher expression in serum and two showed higher expression in serum-freeB4L (Table 3 and Figure 4).
The minimal cut-off for the search was chosen at a score of 0. The choice of this rather low threshold permits the detection of all ZFs/ZFPs, but also results in the detection of many false-positives.
Furthermore, our median sequence coverage of 41× (minimal cut-off of 10 reads) is substantially higher than that found in other studies, in which sequencing depth is usually between 6-16× with lower cut-off values than used in this study [ 30, 32, 33, 42, 57].
An estimated 100,000 to 200,000 Americans subsist in states of partial or minimal consciousness, cut off from those around them.
The minimal codon usage cut off was set at 2% and the ΔG optimization was set to -15 to -25 kcal/mol.
But as the influx accelerated, Lebanon's government cut off minimal cooperation with the understaffed United Nations refugee agency, which struggled to track scattered Syrians.
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