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Inspiratory-inhibited neurons exhibit significantly more positive resting membrane potential, more negative voltage threshold and lower minimal current required to evoke an action potential under current clamp.
The aim of this study was to determine the minimal current required to stimulate a nerve while different pulse durations were applied, and to evaluate the importance of the placement of the cutaneous electrode.
For in vitro electrophysiologic studies, an unpaired t-test was used to compare the resting membrane potential, input resistance, and minimal current required to evoke spikes.
Neuronal spiking response was tested by injecting brief (1 sec) depolarizing current pulses (100 400 pA) and measuring total number of spikes evoked, the minimal current required to evoke spike, and the maximum peak frequency of the evoked spikes.
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In order to standardize the analyzed profiles across all neurons, the stimulus strength was normalized according to the minimal step current required to reach AP-threshold (the membrane potential above which an action potential is initiated).
The decreased spiking response was accompanied with a higher minimal depolarizing current required to evoke spikes and a lower peak discharge frequency.
The decrease in spiking response was accompanied with a higher minimal depolarizing current required to evoke spikes (FA, 155 ± 21 pA; PM, 223 ± 23 pA; p = 0.041).
We determined the minimal level of current required to elicit the following stereotypical responses: flinching, running, vocalization, and jumping.
To exclude a possibility that a different nociceptive response might contribute to the difference above, the minimal amount of current required to produce stereotypical behaviors (flinching/running, jumping, and vocalizing) was measured after the retention tests, and the results did not show any significant difference between these mice (data not shown).
No current requires small split shots.
The average minimal depolarizing current amplitude required to elicit spike discharge (rheobase) was significantly lower for MSN in dopamine-depleted animals (62.5±6.2 pA, n = 8) than the current to threshold for neurons in untreated control animals (91.25±2.95 pA, n = 8, p<0.05; Fig. 2C).
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