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However, as sequencing technologies improve critical factors such as read length, it would be prudent to reassess minimal coverage required for accurate assembly.
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We evaluated the minimal sequencing coverage required to obtain a full-length, high-quality genome using in silico assembly experiments.
Using a random data sampling approach, the minimal genome coverage required for the FindInsertSeq workflow to provide reliable results was determined to be 22× fold.
The minimal sequencing coverage required in F. graminearum to survey accurately the genome to a single base level for recombination events was determined to be 22× fold.
For haploid genomes, the minimal coverage depth required is lower.
Find out if your state requires minimal coverage only, or if you need more comprehensive coverage, including uninsured motorist coverage.
The ability to estimate cross contamination levels would then be valuable in reporting the level of background noise, enabling confidence levels to be ascribed to observations and analysis settings (e.g. required minimal coverage) to be modified.
Thus we required a minimal coverage of 4-fold for homozygous SNPs and 8-fold for heterozygous SNPs.
Like the identification of NCO GCs, the identification of spontaneous mutations required a minimal coverage of 10, an allele frequency higher than the cutoff to distinguish between homozygous and heterozygous positions (as defined for the tetrad analysis), and equivalent evidence for the non-mutant allele in the other samples.
Ultimately, the cost of this approach is determined by the minimal coverage, which in turn determines the sensitivity required for the detection of potential sequence variants.
The trade-off is that young people would no longer be permitted to bet on their good health: All the reform legislation before Congress would require individuals to buy at least minimal coverage.
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