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Specifically, MAQ's minimal map quality for the read, minimal consensus quality and minimal map quality of the best mapping read for each predicted SNP position were used as criteria to select reliable SNPs by setting the thresholds for all three parameters at 10 (SNPs with any values<10 were discarded).
Further criteria used to exclude less reliable SNPs from the dataset included a minimal map quality for the read of 10, minimal consensus quality of 10 and a minimal map quality of the best mapping read of 10 for each predicted SNP position.
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SNPs were filtered using maq.pl SNPfilter with minimum read depth (-d) of 20, minimum consensus quality (-q) of 20, and minimum adjacent consensus quality (-n) of 20.
We filtered the MAQ [ 30] SNP output according to several rules: minimal map quality per read: 10; minimal map quality of the best mapping read on a SNP position: 10; maximum read depth at the SNP position: four times the actual coverage after quality filtering; minimum consensus quality: 10 [ 22].
Open image in new window Fig. 1 Collaborative algorithm using consensus quality.
Furin cleaves a wide range of proproteins at the consensus sequence Arg-Xaa-Lys/Arg-Arg-↓, and the minimal consensus sequence for Furin is Arg-Xaa-Xaa-Arg-↓.
The NFAT minimal consensus sequence is GGAAA A) or TGGAAAGT.
The minimal consensus ERE sequence is a 13 bp palindromic inverted repeat: 5'-GGTCAnnnTGACC-3' [52,53].
These resources generally had a low risk of bias, but there was minimal consensus among them.
Events (losses or gains) selected as diagnostic characters corresponded to minimal consensus regions.
The flanking regions around the minimal consensus sequence in the PCSK targets are less well examined.
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