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This assay assesses the potency of antibiotics by determining the minimal concentration at which no visible bacterial growth can be observed.
The concentration of 2-AP used was based on the titrations carried out by Cromie and Leach [7] and 20 µg/ml was chosen as a minimal concentration at which clear effects on viability could be observed.
The detergents were all tested at a total concentration of 2%, since this was the minimal concentration at which these detergents provided partial transient protection.
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Angiostatic activities are represented by the minimal concentrations at which each bee products suppresses all of parameters against VEGF-induced tube formation.
Table 3 shows the minimal concentration of IPTG at which still some growth was observed for the different conditional mutant strains.
The minimal concentration of the antimicrobial at which 100% inhibition of growth was observed (MIC100) was determined by measuring the absorbance at 600 nm (Biorad microplate reader model 680, Hercules, CA).
In conclusion, after subcutaneous injection of a therapeutic dose in glargine-treated type 2 diabetic subjects, glargine is only transiently, and at minimal concentration, detectable in plasma, whereas its metabolite M1 accounts for most (>90%) of the plasma insulin concentration.
When applied at the minimal concentration needed for brief partial protection, all five detergents caused a subsequent increase in susceptibility at 12 hours of ~20 30-fold ~20 30-fold
The methanol extracts showed high inhibition at the minimal concentration for most of the clinical pathogens in comparison to other extracts.
The factors in the CLiMax model are conditional probabilities that the probe intensity will exceed a certain threshold, given the presence of a particular target at some minimal concentration.
The following day, G418 at the predetermined minimal concentration that was completely lethal to neomycin-sensitive cells was added to initiate the selection of neomycin-resistant (i.e. transduced) cells by elimination of nontransduced cells with that concentration of G418.
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