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In this article, we report further refinements to obligate heterodimer variants of the FokI cleavage domain for the creation of custom ZFNs with minimal cellular toxicity.
We established the optimal NaBt concentration for PC12-ND6 cells (5 mM) and E17.5 hippocampal neurons (2 mM) to induce maximal acetylation levels with minimal cellular toxicity, and showed that NaBt preferentially acetylated the lysine residues of the histone H3 at positions 9 and 27 (Fig. S1).
Figure 5 Magnetic nanospheres have minimal cellular toxicity.
We used As2O3 at 5µM with 5µM MG132 which elicited minimal cellular toxicity compared to higher doses.
Two scans were used to generate DNA damage restricted to the laser path with minimal cellular toxicity.
Another major confounding factor is the specific targeting of the anti-HIV gene to have maximal effect against HIV-1 coupled with minimal cellular toxicity.
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An established method for imaging deep into living tissue, while inducing minimal fluorophore bleaching and cellular toxicity, involves two-photon laser scanning (2P) microscopy [39].
In a program aimed at the identification of small molecule inhibitors of the canonical Wnt pathway we identified a series of 2-aminopyrimidine derivatives which specifically inhibited the pathway with minimal or no sign of cellular toxicity.
Oh, E. et al. Meta-analysis of cellular toxicity for cadmium-containing quantum dots.
Fusco, G. et al. Structural basis of membrane disruption and cellular toxicity by alpha-synuclein oligomers.
H3.3K36M-associated H3.3K36M-associatedhenocellularhe specific depletoxicityH3K36me2, but not loss of H3K36me3.
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minimal systemic toxicity
minimal cellular decomposition
minimal cellular infiltration
minimal cellular damage
minimal renal toxicity
minimal cellular disruption
minimal cellular structure
minimal cellular machinery
minimal cellular function
minimal cellular internalization
minimal cellular content
minimal cellular uptake
minimal cellular response
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