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Given these results, it was possible to determine the putative effects of B [a]P exposure on plasma cell differentiation during the second culture under conditions of minimal cellular proliferation.
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In control cells, citrate treatment had minimal effect on cellular proliferation (0 to 15% reduction in thymidine incorporation), while in SCD1-deficient cells the decrease was ≥30% with respect to vehicle-treated cell counterparts.
Importantly, treating Huh-7.5 cells or primary human hepatocytes with recombinant BMP4 resulted in a dose-dependent increase in the number of infected cells (Fig. 3D) and HCV RNA levels (Fig. 3E,F) with minimal effect on cellular proliferation.
Although a lengthening of doubling time of DKC1-depleted MEFs by both targeting shRNA hairpin was observed, compared to shDKC1-1, knockdown by shDKC1-2 hasignificantlyntly more pronounced effect on iPS cell generation while having a minimal impact on cellular proliferation.
Moreover, Hashimoto et al. have recently demonstrated that some tissue-resident macrophages repopulate locally via cellular proliferation with minimal contribution from monocytes, but these findings were observed under steady-state situation in alveolar macrophages, which are known to possess unique features such as a half-life 3 to 4 times longer than other macrophage subsets [ 34].
The duration of illumination chosen for the combination experiments was 2 min, which is a compromise between minimal level of cytotoxicity in the MGHU1 cell lines and reducing the apparent phenomenon of cellular proliferation, at lower doses, in the MCF-7 cell lines.
The human wart virus causes epidermal cellular proliferation and hyperkeratosis.
These parameters influence the cellular proliferation process.
(We consider with a minimal cellular decomposition).
Cellular proliferation was estimated by incorporation of [3H] thymidine.
Cellular proliferation was assessed using CCK-8 and BrdU Cell Proliferation Detection Kits.
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