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FITC-labeled anti-CA125 MAb remained membrane bound over a period of 48 h with minimal cellular internalization observed upon incubation with live NIH OVCAR-3 cells iNIH OVCAR-3Figure 3). Figure 3 cellscal minroscultureages oFigureOVCAR-3 cells directly immunostained with FITC-labeled anti-CA125 MAb over 48 h.
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Cellular internalization required sulfated proteoglycans and cholesterol.
Duan, X. & Li, Y. Physicochemical characteristics of nanoparticles affect circulation, biodistribution, cellular internalization, and trafficking.
Cellular internalization routes determine the post-internalization fate and intracellular localization of nanoparticles.
Efficient cellular internalization is observed by flow cytometry and fluorescence microscopy, resulting in the inhibitation of cancer cell proliferation.
The cellular internalization and biolocalization of the FITC-labeled HAPNs were also identified.
Additionally, cellular internalization behaviour and viability was found to vary with different cell types.
To enable cellular internalization, cell penetrating peptide from HIV-1 Tat (TAT) was incorporated.
Confocal laser scanning microscopy (CLSM) visualized cellular internalization of the coumarin 6-loaded MPEG PLA NPs.
Multivalent linear DNA structures containing dual types of aptamers showed significantly superior cellular internalization.
Furthermore, the prepared mPEG-PPF-based micelles demonstrated excellent cytocompatibility and cellular internalization in vitro.
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