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Our method provides an alternative way to explore the minimal cellular function set.
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SSMCs are liposome-based cell-like molecular assemblies designed for displaying minimal cellular functions, like gene transcription and translation.
The high Jaccard coefficients between the minimal pathway maps of E. coli and the pathway maps of several parasitic genomes imply that the minimal cellular functions are represented in the minimal pathway maps of E. coli.
Under most conditions, autophagy promotes cell survival by allowing cells to adapt to stressful conditions; thus, cells are provided with the energy required for minimal cellular functions even when nutrients are scarce [8].
Instead of a minimal gene set, we computationally explored a minimal cellular-function set.
We evaluate a minimal cellular-function set directly, instead of a geneset.
Such studies have provided clues to the minimal cellular-function set needed for life.
We consider that they correspond to a minimal cellular-function set.
We consider this methodology provides an alternative way to explore a minimal cellular-function set, other than current experimental and computational approaches.
The pathway maps with high OF values are considered to be the "conserved pathway maps", and they will be the "core" of a minimal cellular-function set.
As mentioned in the Background, a minimal genome and a minimal cellular-function set depend on the environment or nutrients, and their general definitions are very difficult.
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