Sentence examples for minimal cell number from inspiring English sources
Fig. 4 Minimal cell number for lactate measurement in healthy volunteers' T lymphocytes.
Correlation between intra-lymphocyte and supernatant lactate concentration was explored with Pearson correlation 4. Minimal cell number .
To correctly measure this phenomenon, minimal cell number was 250,000 and optimal culture duration was 40 h.
To determine the minimal cell number, S. Typhimurium ATCC 23566 was cultured in lactose broth and incubated at 37°C overnight (16 hr), then subcultured (1% inoculum) in freshly prepared lactose broth, and further incubated at 35°C for 5 hr with shaking.
We have established an optimised RNA isolation workflow specifically designed to yield maximal RNA from minimal cell numbers.
We also demonstrate the successful global transcriptional profiling of biopsies containing minimal cell numbers which have previously been limited to focussed transcriptional interrogation.
Genomic DNA (gDNA) elimination was carried out on the total RNA samples from minimal cell numbers by gDNA Wipeout buffer treatment (Quantitect Reverse transcription kit, Qiagen).
When processing clinical biopsies containing minimal cell numbers good quality cDNA and array metrics were obtained, although a reduction in sscDNA yield was observed.
Following RNA isolation optimisation using either HUVEC titrated minimal cell numbers or vascular endothelial biopsies, 10% of isolated RNA was used for reverse transcription.
When measuring RNA quantity from HUVEC titrated minimal cell numbers, all of the isolated RNA was used in the Quantitect Reverse Transcription Kit (Qiagen).
