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C. elegans was grown axenically in a chemically defined nutrient medium before being transferred to minimal buffer containing a standardized density of bacteria in multiwell plates.
C. elegans strain N2 was grown axenically in CeHR, washed, and transferred to minimal buffer containing a standardized density of bacteria in multiwell plates.
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Both automated protocols were developed to use generic commercially available pre-packed columns and automatically prepared minimal buffers, designed to eliminate user and system variations, maximize run reproducibility, standardize yield and purity between batches, increase throughput and reduce user input to a minimum.
Both strains displayed glycolytic oscillations upon growth in minimal buffered (SD) but not rich (YPD) medium (data not shown).
The Lean paradigm uses approaches that are relatively novel in health care, such as management of minimal inventories, minimal buffers of work-in-process and minimal latitude in planning [ 9].
This was followed by dialysis against minimal phosphate buffer saline pH 7 by (10000 MWCO) and left for 4 h with continuous mixing at 4 °C.
Finally, nematodes were washed in M9 minimal salts buffer and transferred on plates containing the appropriate bacterial strain.
Nematodes were then treated for 1 h with 20 μg/ml Polymyxin B antibiotic in M9 minimal salts buffer.
The CNS was transferred from the Petri dish via pipet, with minimal dissection buffer, to a sample vial containing 10 μL of 0.5 mM perchloric acid.
Two-day old adults were separated from their progeny on the first and second day by gravity sedimentation in 15 ml falcon tubes for 2 min in M9 minimal salts buffer, the larvae being removed with the supernatant.
Nematodes were washed in M9 minimal salts buffer to remove excess bacteria from their surface and then incubated in NGM for 1 h to allow them to expurgate intestinal bacteria.
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