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This suggests that - with these enrichment technologies - the minimal amount of input DNA cannot easily be reduced beyond 500 ng.
We explored the possibility of generating, from a minimal amount of input DNA (≤ 1 ng) in the MDA reaction, genome-equivalent DNA that can be efficiently and accurately typed for a large number of SNPs.
Such efficiency and accuracy of SNP typing of amplified DNA suggest that MDA can also generate large amounts of genome-equivalent DNA from a minimal amount of input DNA.
Using identification of genetic variations in prostate cancer as an example we address three crucial challenges in the field of targeted re-sequencing: Small nucleotide variation (SNV) detection in samples of formalin-fixed paraffin embedded (FFPE) tissue material, minimal amount of input sample and sampling in view of tissue heterogeneity.
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From these observations, we concluded that 10 pg is the minimal amount of P. falciparum input DNA that produces quality genome coverage under the optimized MDA conditions described.
However, the analogue of the theorem fails if it is only assumed that a fixed fraction of regulatory functions shows a specified minimal amount of bias, even when the number of inputs (but not outputs) of each regulatory function is bounded.
The need for direct access to highly relevant, location-aware information with a minimal amount of browsing and a minimal amount of user input is what prompted us to investigate the feasibility of and development of a plan for deploying mobile agents to perform information filtering and retrieval on behalf of the users in a mobile commerce setting.
Figure 2 also shows that the minimal amount of recurrent excitation needed to have decision states depends non-monotonically on the input, as a consequence of the greater recruitment of shared inhibitory feedback for higher input strengths [16].
The minimum heat input is that which will just maintain the temperature at 100 °C with the minimal amount of evaporation of steam.
A minimal amount of cartilage is needed.
We need a minimal amount of time.
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