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As for the YPG who have been trying to carve out a Kurdish mini-region of their own within the conjectured "buffer zone," they would be left out in the cold.
The amount of the fragment spanning the hsp70 promoter and mini-white coding region of the reporter constructs precipitated was determined with real-time PCR employing primers 5W3 (5'-AGT GAA CAC GTC GCT AAG CGA AAG) and 3W2 (5'-GGG ATT TTT GTG GGT CGC AGT TCT).
This plasmid integrates in the highly repressed 177 bp satellite region of a mini-chromosome.
This MS-generating mutation process has also been shown in an internal region of the mini-me elements of Dipterans [ 13].
To address these needs, a 35S promoter driven version of a commonly used TMV expression vector (containing the TMV CP gene) [ 11] was constructed in the T-DNA region of the mini-binary vector pCB301 [ 16].
The primers used to perform the PCR for annotating the genes, together with another 18 optimized primer pairs, amplifying genes not present in the mini-contig but flanking the genomic region of the different RPSA family members, are listed in Additional file 6.
This study was undertaken to improve the stability of αA-mini-chaperone while preserving its anti-aggregation activity by fusing the flexible and solvent-exposed C-terminal 164 173 region of αA-crystallin to the mini-chaperone sequence DFVIFLDVKHFSPEDLT.
Total RNA was extracted from 35 mg of tissue from the stem-root region of P. nigrum using the Illustra RNAspin Mini Kit (GE Healthcare, USA).
Individual blastomeres in the ventro-lateral region of embryos at the 128-cell stage were injected with mini-Ruby (Fig. 1D,E).
Slavonia, historical region of Croatia.
The region of the country?
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