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A glass mini column of 30 cm length and 2 cm internal diameter was used.
Tryptic peptides from whole cell or nuclear protein extracts were loaded onto a 2 g Sep-Pak C18 column (Waters), washed twice with 10mL1%1% acetic acid, eluted with 7 mL 80% acetonitrile containing 0.1% acetic acid, lyophilized using a speed-vac, resuspended in 400 μL 1% acetic acid and loaded onto a mini column of 40 μL IMAC resin (prepared as previously described [ 46, 47]).
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A solid phase extraction procedure has been described for the determination of Cd, Cu, and Pb by High Resolution Continuum Source Flame Atomic Absorption Spectrometry HR-CS FAAS using a mini-column of Mucor pusillus (Lindt., 1886) immobilized on Amberlite XAD-4.
To further remove short RNAs, the subtracted RNA was passed over a mini-column of the RNeasy mini kit (Qiagen).
The adsorption experiments by batch and mini column scale to test the potential of granular ferric hydroxide for removal of various ions including fluoride was studied [49].
In this study, to evaluate the fresh properties of self-consolidating mortars, three apparatus (mini column segregation, mini J-ring and mini Orimet tests) have been developed as a simple and fast methods for testing rheological properties of SCMOs.
Problems in the application of low pressure gas source and mini column are investigated, including column simplification, air liquefaction in flammable MR JT, etc. System exergy analysis is conducted.
Beads were separated magnetically prior to loading of the suspension on the RNA capturing mini column.
The resulting aqueous-ethanol solution was loaded onto an RNeasy mini spin column of the Qiagen RNeasy Mini Kit (Qiagen).
Supernatant after chloroform treatment was mixed with an equal volume of 70%% (v/v) chilled ethanol before loading onto the QIAGEN RNeasy mini column.
The entire sample was then loaded into a mini spin column (QIA shredder; Qiagen) and centrifuged in a mini-centrifuge (Beckman Coulter, Mississauga, Ontario, Canada) for 2 min. Supernatants were collected, washed with 0.25 mL of 100% ethanol, and loaded into a silica membrane mini-column (RNeasy mini column; Qiagen).
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