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The bone volume fraction and average tissue mineral density, or the degree of mineralization, were calculated for the entire core (subchondral plate and subchondral trabecular bone).
Based on the measurements, parameters of bone formation and mineralization were calculated [ 47].
Rates of microbial mineralization were calculated using the change in NH+4 and NO−3 [incubated (NH+4 + NO−3) − initial field (NH+4 + NO−3)] and nitrification rates were calculated from the change in NO−3 (incubated NO−3 − initial field NO−3).
Similar(57)
Percent callus mineralization was calculated as mineralized callus volume divided by total callus volume and normalized against vehicle (PBS) or IgG isotype control.
The concentration of mineralization was calculated based on the standard graph depicted using the absorbance of standard samples.
The cumulative SMZ mineralization was calculated from the mineralization rates every day.
The cumulative mineralization was calculated each day from the actual mineralization rates.
Net mineralization was calculated as the change in N H 4 + minus the change in N O 3 − over time, and net nitrification was calculated as the change of N O 3 − over time following Riley and Vitousek (1995).
Furthermore, net mineralization coefficient and complementary mineralization coefficient were calculated from C mineralization data.
Spectra were then processed as described below, and the mineralization ratios were calculated for the ratio of phosphate (η1; 960 cm−1) to amide I and the ratio of phosphate (η4; 588 cm−1) to amide III; of note, the former measure, being a symmetric mode, is more sensitive to polarization (26) (details available from the corresponding author upon request).
The kinetics of degradation was pseudo-first order; the degradation and relative mineralization of the OT were calculated by HPLC and COD tests, respectively.
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