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The mineralization time was controlled at 24 h.
When the mineralization time was increased to 12 h (MAS12), spindly crystals were heavily aggregated.
In order to investigate the effect of AS concentration and mineralization time on the nucleation of HAps, the concentrations of AS were controlled to be 0.5, 2, and 8 mg/mL, and the mineralization time was set at 2, 6, 12, and 24 h, respectively.
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A second phase was conducted, to find out if this approach is sustainable and the mineralization capacity is stable in soil over time.
A potential difficulty is the preferential mineralization of the outermost portion of the enamel that can prevent overall mineralization.
Meanwhile, mineralization lag time was increased 5- to 6-fold in the CKO groups (Figure 7F).
The mineralization lag time was higher in AIRF group.
Mineralization lag time is expressed in days.
Based on the SOC mineralization rate, the cumulative mineralized SOC for a specific period of time was also calculated following the empirical function below.
The decalcification time was variable (minimum 3 days, maximum 3 weeks), depending on the gestational age and, implicitly, on the mineralization degree.
These experimental results were predicted by a theoretical model combining mineral growth kinetics with fracture energetics, suggesting that toughness increased with mineralization time until mineral mobility was attenuated by steric hindrance, then returned to baseline levels following the rigid percolation threshold.
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