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According to statistical analysis, the initial pH, inoculum size, incubation time and mineral solution were the insignificant factors with low confidence levels (<90 %) for all responses, except mineral solution in the LD model (Table 4).
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However, this factor gave a negative effect on LD, which means that if a lower mineral solution was added LD could be improved.
The garden relies on a technique called aeroponics where water and a mineral solution are used as a base instead of soil, allowing plants to thrive in smaller, more contained environments.
The mineral solution was changed once a week.
Afterwards, the mineral solution was prepared using the corn cob extract enriched with 1% glucose as carbon source.
In the experiments of adding a mineral solution, the following mineral solution was used to replace distilled water (L−1): (NH4)2SO4 1 g, KH2PO4 0.5 g, K2HPO4 0.5 g and MgSO4 0.2 g.
More promisingly, when a mineral solution was added, the cellulase activities achieved 16.7 and 24.0 U/g after 3 and 5 days culture, respectively.
For anaerobic growth of S. oneidensis MR-1 and its mutants, the defined medium M1 [per liter: 1.19-g (NH4 2SO4, 0.56-g KH2PO4, 0.1 ml (0.115 M) Na2SeO4 and 10 ml 10X mineral solution) was used as described previously [ 30].
To create a gradient in mineral along the scaffold length, the as-prepared mineral solution was loaded into a 50 mL plastic syringe and fed continuously into a glass vial using a syringe pump at a feeding rate of 36 mL/h.
Molasses and mineral solutions were autoclaved separately.
Sterile aliquots of the vitamins and mineral solutions were added after the mineral salts medium was autoclaved at 121°C for 20 min. Filter sterilized sodium bicarbonate (10 mM final concentration) was added to provide buffering capacity and a carbon source.
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