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However, this factor gave a negative effect on LD, which means that if a lower mineral solution was added LD could be improved.
The mineral solution was changed once a week.
Afterwards, the mineral solution was prepared using the corn cob extract enriched with 1% glucose as carbon source.
More promisingly, when a mineral solution was added, the cellulase activities achieved 16.7 and 24.0 U/g after 3 and 5 days culture, respectively.
In the experiments of adding a mineral solution, the following mineral solution was used to replace distilled water (L−1): (NH4)2SO4 1 g, KH2PO4 0.5 g, K2HPO4 0.5 g and MgSO4 0.2 g.
For anaerobic growth of S. oneidensis MR-1 and its mutants, the defined medium M1 [per liter: 1.19-g (NH4 2SO4, 0.56-g KH2PO4, 0.1 ml (0.115 M) Na2SeO4 and 10 ml 10X mineral solution) was used as described previously [ 30].
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According to statistical analysis, the initial pH, inoculum size, incubation time and mineral solution were the insignificant factors with low confidence levels (<90 %) for all responses, except mineral solution in the LD model (Table 4).
The garden relies on a technique called aeroponics where water and a mineral solution are used as a base instead of soil, allowing plants to thrive in smaller, more contained environments.
Molasses and mineral solutions were autoclaved separately.
Hydroponics is the practice of growing plants without soil, using water as the base because mineral solutions are what are vital to plant growth, not soil itself.
Sterile aliquots of the vitamins and mineral solutions were added after the mineral salts medium was autoclaved at 121°C for 20 min. Filter sterilized sodium bicarbonate (10 mM final concentration) was added to provide buffering capacity and a carbon source.
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