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This study conducted a direct comparison of mineral affinity of BP protein conjugates linked together with cleavable disulfide and non-cleavable thioether linkages.
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Except for the selectivity of mineral affinity to metals, pH value has been reported as an important factor to influence competition.
Chemical conjugation of bisphosphonates (BPs) to therapeutic proteins is an effective means to impart mineral affinity to proteins.
Although all are pyrophosphate analogs and reduce bone reabsorption, they differ from each other in terms of their mineral affinity and biochemical action on bone cells.
The imparted mineral affinity, as assessed by hydroxyapatite binding in vitro, was lost upon the cleavage of the disulfide-linked aminoBP.
It is noteworthy that BSF and HSA that had been trapped by precipitating minerals gave similar intensities when analyzed by SDS-PAGE in spite of a 10-fold difference in their concentrations used, indicating again a much greater affinity of these mineral complexes for fetuin-A as compared to albumin (see Fig. 21 of ref. [2]).
The high sorption affinity of TPP on mineral surfaces reduce the risk of TPP moving offsite or into untargeted areas causing unintended P-related ecosystem damage.
The absence of the Hg species in this PC is in agreement with the higher affinity of Hg for acidic minerals compared to basic minerals as demonstrated by Hg determinations in such samples [42].
The grafted zwitterionic polymer brushes not only effectively templated the surface mineralization, increasing the surface mineral coverage by >100% from those achieved with unmodified Ti6Al4V substrate, but also significantly improved the bonding affinity of the surface apatite minerals to the metallic substrate.
Acid mine drainage (AMD) is often accompanied with elevated concentrations of arsenic, in the forms of arsenite, As III), and/or arsenate, As V), due to the high affinity of arsenic for sulfide mineral ores.
Mechanisms of binding and the relative binding affinity of various BPs to bone mineral are introduced, including common methods for measuring binding affinity in vitro and in vivo.
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