Sentence examples for minced into approximately from inspiring English sources

The tissue samples were washed extensively with phosphate-buffered saline (PBS), minced into approximately 1-mm3 cubes using scissors and dissociated into single cells by digestion with collagenase (Wako Pure Chemical Industries).

The excised pancreas was then minced into approximately 1 mm3 pieces and digested at room temperature for 1 hr in the dissociation medium (Ringer solution supplemented with 0.2 mg/ml soy beans trypsin inhibitor (Sigma), 2 mg/ml BSA (Roche), 400 U/ml hyaluronidase (Sigma) and 50 U/ml collagenase (Sigma)).

Tumor tissue was minced into approximately 1.0~2.0 mm3 fragments and suspended in 2 mL of normal saline, then drawn into a 2 mL injector.

Retinal and brain tissue was dissected (minced into approximately 2-mm pieces) and suspended in 3 mL homogenization buffer (20 mM tricine, 25 mM D-sucrose, 15 mM NaCl, 60 mM KCl, 2 mM MgCl2, 0.5 mM spermidine, pH 7.8).

Zymbal glands were excised bilaterally from the ear ducts of female Sprague-Dawley rats (three/group), minced into approximately four fragments per gland, and transferred into a microtiter plate containing 1.5 mL per well of Waymouth's tissue culture medium supplemented with fetal calf serum, hydrocortisone, insulin, and gentamicin.

The collected ligaments were minced into approximately 0.5-mm3 pieces and then plated onto 25-mm2 culture dishes and maintained in Dulbecco modified Eagle medium supplemented with 10% fetal bovine serum, 100 unit/ml penicillin G sodium, and 100 μg/ml streptomycin sulfate in a humidified atmosphere of 95% air and 5% CO2 at 37°C.

Specimens were mechanically minced into pieces approximately 0.5 1 mm3 in size using a sterile scalpel, under aseptic conditions in a laminar flow hood, washed again and incubated in "standard medium" DMEM/F12 1:1 containing 1% Penstrep, 1% ITS and 0,1% Gentamycin with 200 U/ml Type II Collagenase (Gibco/Invitrogen, Prague, Czech Republic) for 4 hours at 37°C.

The clear muscle tissues were then minced into pieces of approximately 1 × 1 × 1 mm and transferred into culture flask (Corning, USA), supplemented with 2 mL of DMEM containing 15% FBS and 100 U/mL penicillin-streptomycin.

Tumour tissue was minced into pieces of approximately 1 mm3 and incubated for 20 min at 37°C in PBS containing 2.5 mg/ml collagenase I, 0.25% trypsin, and 0.01 mg/ml DNase (all from Sigma, Dorset, UK).

For treatment by nutlin-3 (Biomol, Hamburg, Germany) tissue samples were minced into small pieces of approximately 0.5 cm diameter and incubated in medium 199 supplemented with 20% FCS and nutlin-3 (3, 10, or 50 μM) for 72 h.

NP tissues were finely minced into small fragments of approximately 2 mm and digested with 0.02 % w/v pronase (Sigma-Aldrich) (37 °C, 5%% CO2, 1 h) in 20 ml DMEM (Dulbecco's Modified Eagle's Medium), containing 1 % penicillin/streptomycin, 1 % glucose and 10%% FCS (fetal calf serum) (Sigma-Aldrich).