Sentence examples for min with slow from inspiring English sources

Formaldehyde was added to a final concentration of 1% and incubate at RT for 10 min with slow mixing.

The cell suspensions were mixed with 40 mg of hydroxyapatite/tricalcium phosphate (HA/TCP) ceramic powder (particle size 0.5 1.0 mm, generously provided by Zimmer, Inc., Warsaw, IN), and the mixtures were incubated at 37°C for 90 min with slow rotation (25 rpm) prior to implantation.

T4 DNA Ligase (100 U, Fermentas, Vilnius, Lithuania) was then added, and DNA was ligated at 14-16°C 14-16°C forlowed by further incubation at room temperature for 30 min with slow agitation.

Ribosomes were also analyzed on 10 30% gradients prepared in 50 mM Hepes, pH 7.4, 2.5 mM MgCl2 and 100 mM KOAc using the SW55Ti rotor at 50,000 rpm for 100 min with slow acceleration /deceleration.

The cells were lysed in a buffer containing 1 × PBS (MP Biomedicals, France) supplemented with 1 mM EDTA, 0.1% Triton X-100, 1 mg/ml lysozyme and placed on ice for 30 min with slow shaking before sonication.

The tissue was cut into 5 mm pieces and epithelial cells released by two cycles of treatment with 1 mM EDTA and 1 mM dithiothreitol (DTT) in HEPES-buffered Hank's balanced salt solution containing 2% of fetal calf serum at 37°C for 15 min with slow stirring (Lundgren et al., 2005).

In both groups, Tc-99m-teboroxime cleared rapidly from the blood during the first 5 min, with slower clearance over the remaining period of study.

Indeed, the rapid rise in plasma biopterin (observed within 20 min) coupled with slow GCH1 GFRP dissociation rates (obtained by SPR) supports the view that GCH1 and GFRP are likely to be constitutively bound in vivo, rather than binding in response to an acute elevation of circulating L-phe, following dietary intake.

Due to the presence of the coating agents, the release of SB from coated samples could still be observed even after 3500 min with a slow and sustained release characteristic.

Add 40 μl of Zymolyase solution and 40 μl of ME into the tube, mix wel,l and incubate at 30°C for ~ 20-40 min with a slow shaking.

The annealing reaction was carried out for 2 min at 80°C with slow cooling to 25°C.