Sentence examples for min with several from inspiring English sources

Next, we explained "concept map" and "method of making the concept map by the system" in 10 min with several examples.

In brief, DNAs were subject to repeated denaturing (99°C for 5 min) with several incubation steps (60°C for 25 min, 85 min, and 175 min).

The tube was placed in a heat-block and incubated at 63°C for 20 min with several vigorous shakes in between.

The coverslip was removed together with the rubber cement seal, and the slide was immediately placed into a 0.4× SSC/0.3% NP-40 wash solution at 73° for 4 min, with several agitations per minute.

Primary antibodies were detected by HRP (horseradish peroxidase -conjugated secondary antibodies (DakoCytomation) diluted by 1:5000 in blocking buffer containing 3% (w/v) BSA in TBS (Tris-buffered saline), followed by washing with TBS for a total of 30 min with several changes.

The reaction was stopped after 5 min by washing with several large volumes of deionized water.

Changes seen in later (60 min) ERK activation with several estrogen/XE combinations may indicate functional consequences for regulating cell proliferation (Razidlo et al. 2004; Yang et al. 2008) and other epigenetic aspects of carcinogenicity (Bredfeldt et al. 2010).

Phytochemical studies using high performance liquid chromatographic (HPLC) fingerprint profile of the MeOH extracts of noni fruit revealed three major peaks representing scopoletin, rutin and quercetin (retention times: 25.72, 28.57 and 32.70 min, respectively), together with several minor peaks.

The selected seeds of peanut (Arachis hypogaea L). were surface sterilized with 5%H2O22 for 30 min, washed several times with sterile deionized water, and germinated on wet sterile sand at 25°C in the darkness.

Kinetic studies showed that RAW264.7 cells stimulated with M-CSF from 15 min to several days with media change every two days did not reveal any differences in the subcellular localization of Mitf (data not shown).

For BrdU detection, immunostained tissue sections were postfixed for 15 min with 4% PFA in phosphate buffer, washed in TBS, incubated in 2 M HCl for 30 min at 37 °C, and neutralized with several washes with TBS.