Sentence examples for min with regular from inspiring English sources

The samples were incubated for 30 min with regular vortexing and were subsequently centrifuged at 21 000 × g for 15 min.

Briefly, the cells were incubated for 30 min with regular culture medium supplemented with 2 μM calcein AM and 4 μM EthD-1 and analyzed via fluorescent microscopy (Leica DMI6000, Buffalo Grove, IL).

For analysis of gastric glands, gland preparations were fixed in 4% (v/v) PFA in PBS at room temperature for 20 min, with regular re-suspension of the glands.

Briefly, 2 g of test samples were taken in a beaker and 50 mL of petroleum ether was added and heated gently on water-bath to 40°C for 5 min with regular shaking.

For FACS, the entire cell suspension was pelleted, resuspended and preincubated in PBS/1% BSA for 30 min on ice with regular mixing (blocking).

The mixture was incubated 30 min at room temperature with regular mixing and then centrifuged.

3 The UK College of Emergency Medicine recommends that patients in severe pain should receive analgesia within 20 min of arrival in the ED, with regular reassessment and further action as required.

Extracts were pre-cleared by centrifugation at 15,000 × g for 15 min. Supernatant was diluted three times with regular IP buffer (25 mM HEPES 7.6, 12.5 mM MgCl2, 100 mM KCl, 0.1 mM EDTA, 0.01% Triton X100).

Finally patients were encouraged to supplement their training with regular home self-practice, of 7 10 min per day (an amount typically recommended in studies of this type).

Free, with regular admission.

He has continued with regular outpatient therapy.