Sentence examples for min with primary from inspiring English sources

Lung sections were then incubated for 40 min with primary antibodies diluted in species-specific serum at a concentration that had been optimized previously.

Fixed specimens were incubated for 40 min with primary antibodies and then for 40 min with TRITC- or Alexa488-conjugated secondary antibodies.

Sections were stained at 37°C for 30 min with primary antibody (ab14356, Abcam Inc) diluted 1∶30 with phosphate-buffered saline containing 0.2% Triton X-100.

Briefly, cells were incubated on ice for 40 min with primary antibody before washing twice with cold PBS containing 1% FCS, then subsequent incubation with various secondary reagents or secondary antibodies [19].

Cell monolayers were fixed/permeabilized in 100% methanol (−20°C for 20 min), blocked in HEPES-buffered Hanks balanced salt solution (HBSS+) containing 1% bovine serum albumin (blocking buffer) for 60 min at room temperature and incubated for another 60 min with primary antibodies diluted in blocking buffer.

Cells at a concentration of 2.5×106 per 100 µl of 2% bovine serum albumin (BSA) were incubated on ice for 30 min with primary antibodies: 0.5 ng/ml of biotinylated TER119 (cat# 553672), CD31 (cat# 558737), CD45 (553078), CD24-phycoerythrin (PE) (cat# 553262), and 0.25 ng/ml of CD49f-allophycocyanin (APC) (cat# FAB13501A, Minneapolis, MN, R&D Systems).

The tissue was then incubated for 60 mins with primary antibody (1 100), washed 3× in blocking buffer and then incubated for 60 mins in each secondary antibody (1 100) and left in blocking buffer.

Briefly, sections were blocked with 4% BSA for 30 min at 37°C and incubated with 3% hydrogen peroxide for 5 min at room temperature (RT) prior to incubation (60 min, RT) with primary antibodies.

Cells were fixed with 50% methanol plus 50% acetone for 2 min, blocked in 1% BSA/PBS/Tween 20 (0.05%) for 30 min, incubated with primary antibody for 1 h and then secondary antibodies for 1 h.

For immunofluorescent staining, frozen skin sections (8 µm) were fixed in 100% acetone at −20°C for 10 min, incubated with primary antibodies at 4°C overnight, followed by AlexaFluor secondary antibodies (Invitrogen), and were analyzed under a DeltaVision microscope.

Cells were blocked with animal-free blocker (Vector Laboratories) for 30 min, incubated with primary antibodies for 1 h, and then washed three times with PBS.