Sentence examples for min with final from inspiring English sources

The radiochemical synthesis of both compounds was completed within 60 min with final high-performance liquid chromatography purification included.

Similarly, in another beaker, 3 mM of thiourea was taken in 30 ml of DIW and stirred for 20 min with final molar ratio of Mo:S precursor being 1 3.

The PCR reaction was initiated at 95 °C for 5 min followed by 30 cycles of 95 °C for 1 min, 55 °C for 30 s and 72 °C for 1 min and a final elongation at 72 °C for 10 min with final hold at 4 °C.

PCR reaction consisted of initial denaturation at 94 °C for 1 min, followed by 30 cycles consisting of 94 °C for 30 s for denaturation, 56 °C for 30 s for annealing and 72 °C for 30 s for extension, a final extension at 68 °C for 7 min with final hold at 4 °C.

The PCR condition was 2 min at 94°C, followed by 40 cycles at 94°C for 15 sec, x°C for 30 sec and 68°C for y min, with final elongation of 5 min at 68°C.

The PCR was initiated at 95°C for 15 min, followed by 45 cycles of denaturation at 95°C for 20 s, annealing at 65°C for 30 s and extension at 72°C for 1 min with final incubation at 72°C for 3 min. 75 cycles were performed in the primer extension reaction for CSHL1.

Reactions were initiated at 95°C for 5 min, followed by 40 cycles of 94°C for 1 min, 59°C for 1 min, 72°C for 1 min and a final extension step at 72°C for 7 min, with a final hold at 10°C in a thermal cycler (Mastercycler gradient, Eppendrof, Germany).

Samples were then amplified as follows: 98 °C for 3 min, 25 cycles of 98 °C for 15 s, 67 °C for 20 s, 72 °C for 6 min, with a final extension at 72 °C for 5 min followed by a 4 °C hold.

Samples were then amplified as follows: 98 °C for 3 min, 14 cycles of 98 °C for 15 s, 67 °C for 20 s, 72 °C for 6 min, with a final extension at 72 °C for 5 min followed by a 4 °C hold.

The RT-PCR program was as follows: 50 °C for 30 min, 95 °C for 15 min, 35 cycles at 94 °C for 1 min, 55 °C for 1 min and 72 °C for 1 or 2 min with a final 72 °C extension for 10 min.

The prepared agar was autoclaved at 121 °C for 15 min with the final pH at 7.3 ± 0.1.