To block endogenous alkaline phosphatase activity, slides were immersed and stirred gently in 0.1 M ethanolamine and 2.5% acetic anhydride for 10 min, followed by treatment with 5 μg/ml proteinase K for 3 min after extensive washing with PBS.
A final notable attribute of the MTD is that the thrombectomy procedure time is approximately 2 to 6 min, without extensive preparation time as with other thrombectomy devices.
Wheat seeds were surface-sterilized with 75% ethanol for 1 min, followed by 1% sodium hypochlorite for 30 min, and extensive wash with sterile water (Collavino et al. 2010).
Odorants were diluted in ND96 and applied for 20 s at a flow rate of 1.65 ml/min with extensive washing in ND96 (7–20 min at 4.6 ml/min) between applications.
Unless otherwise noted, pheromones were diluted in ND96 and applied for 20 sec at a flow rate of 1.65 ml/min with extensive washing in ND96 (10 min at 4.6 ml/min) between applications.
Cover slips (no. 1.5) were sonicated for 20 25 min in acetone, then 1 M KOH with extensive water rinsing between sonication steps.
After washing with TBST, the membranes were incubated with goat anti-mouse IgG (or IgM) or rabbit IgG conjugated with horseradish peroxidase (1∶3000) for 45 min. After extensive washing with TBST, the signal was visualized with SuperSignal west pico chemiluminescent substrate (Thermo Scientific).
Various temperature/irradiation time combinations are investigated, with extensive surface coverage by the graphene obtained after microwave annealing at 1700 °C for just 1 min.
Recombinant FLAG TPL-230 397 and FLAG TPL-230 3972–StrepII–Hand105 complex was puriFLAG TPL-2 ABIN-2 StrepII HA p105s with ANTI-FLAG TPL-2 ABIN-2 StrepII HA p105icomplex 60 min, followas by extensive washing with lysis buffer and elution with 0.2 mg/ml 3×FLAG purified(Sigma–Aldrich) in DM byffer.
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