Sentence examples for min with equal from inspiring English sources

After this, the cells were incubated for 1 min with equal amounts of dye for plasma membrane labeling (1 ml).

Thereafter, work rate was increased every 2nd min with equal increments (10 25 W) throughout the test.

Cells were uncoupled from the magnetic beads by incubating at 37°C for 15 min with equal volumes of isolation buffer and DETACHaBEAD CD34 liquid.

For reducing SDS-PAGE, cross-linked samples were boiled for 3 min with equal volume of SDS-PAGE loading buffer (Biorad, Hercules, CA) containing 1 20 dilution of β-mercaptoethanol (Biorad).

In another set of experiments MCF-7 cells were treated for 30 min with equal doses (5 and 20 μM) of Rottlerin or the chemical uncoupler FCCP, before incubation with 10 μl of MTT for 1 h at 37°C.

Protein spots with significant alterations in expression were manually excised from 2-DE gels, destained for 20 min with equal volumes of 30 mM potassium ferricyanide and 100 mM sodium thiosulfate at room temperature, and washed with Milli-Q water until the gels were destained.

After being boiled for 5 min, samples with equal amounts of total proteins were fractionated by 4 20% precast gradient gel (Bio-Rad).

Whole cell extract prepared from each strain was incubated at 37°C (the non-permissive temperature for the Prp3 ts variants) for 30 min, diluted with equal volume of G100 buffer (20 mM HEPES-KOH, pH 7.0, 100 mM KCl, 0.2 mM EDTA) and fractionated by ultracentrifugation on a 12 ml 10 30 % (v/v) glycerol gradient in G100 buffer.

Protein transduction occurs in a concentration-dependent manner, achieving maximum intracellular concentrations in less than 5 min, with nearly equal intracellular concentrations between all cells in the transduced population.

Samples of sputum were homogenized by incubation at 37°C for 15 min with an equal volume of 0.1% dithiothreitol (Sputolysin; Calbiochem Corp., San Diego, CA, USA).

Sputum samples were shaken for 30 min with an equal volume of 1.0% acetylcysteine and 0.9% sodium chloride, followed by isolating supernatant by centrifuging (10,000 g x 3 min).