Sentence examples for min with cooling from inspiring English sources

Samples were vortexed (3×5 min with cooling on ice in between) in Mixer Mill MM 300 (Retsch).

Cell pellets were resuspended in 100 mM NH4HCO3, pH 8.4 buffer and lysed by using 0.1 mM zirconia/silica beads in a 2.0-ml Cryovial with vigorous vortexing for a total of 3 min with cooling steps.

Cells were lysed by three cycles of homogenisation for 1 min with cooling between each cycle in an ice-water bath for 1 min.

Consecutively, cells were disrupted by bead beating with a MiniBead Beater cell homogenizer (Biospec Products, Bartlesville, OK) and zirconium beads (0.1 mm diameter; Biospec Products) six times for 1 min (with cooling on ice between treatments).

An equal volume of glass beads was added and the cells were lysed by vortexing for 7 min with cooling on ice for 1 min between each vortexing step.

Antigen retrieval was in citrate buffer (pH 6) using an electric pressure cooker for 5 min at 120°C with cooling for 10 min before immunostaining.

Significant recrystallization ability from the melt (at 450°C for 1 min) is observed with cooling rates faster than 200°C/min necessary to quench the polymer into an amorphous state.

After incubating for 30 min at 37°C, cells were mechanically lysed with a XL-2020 sonicator (Misonix Inc. Farmingdale, NY, USA) at 50 watts, five times for 1 min with a cooling step on ice for 5 min between each sonication step.