Sentence examples for min with continued from inspiring English sources

All samples were incubated with sample buffer [0.75 M Tris-HCl pH 8.0, 2% SDS, 0.01% Bromophenol blue, 60% glycerol, 100 mM DTT] at 95°C for 10 min with continued reduction at 37°C for 2 h.

In the H1437 and MDA-MB231 lines concurrent inhibition of PI3K and MEK for 15 min with continued PI3K inhibition for 72 h achieved similar cytotoxicity to concurrent inhibition for 72 h.

The polyethylene glycol solution was then diluted by slow addition of first 1 ml warmed serum- and phenol red-free medium over 3 min with continued stirring and then another 10 ml of this medium over 5 min. After centrifugation cells were resuspended in phenol red-free RPMI 1640 supplemented with 10% FCS.

The CXL was performed according to an established protocol, as follows: corneal epithelial removal, 0.1 % riboflavin eye drops (MedioCROSS H Riboflavin > 0,1 %, Medio-Haus Medizinprodukte GmbH, Kiel) every two minutes for 30 min, and UVA 370 nm at 3 mW/cm for 30 min with continued application of 0.1 % riboflavin eye drops every two minutes.

Thermal cycling for both analyzed genes and GAPDH was initiated with an incubation step at 50°C for 2 min, followed by a first denaturation step at 95°C for 10 min, and continued with 40 cycles of 95°C for 15 s, 60°C for 1 min.

Digestion was conducted with power of 800 W at 110 °C for 15 min, after deflation, continued with power of 800 W at 110 °C for 5 min, and then with power of 1600 W at 160 °C for 15 min.

But instead of stopping the show in a panic, Min gamely continued her performance with partner Alexander Gamelin, improvising as she went so as to prevent her top from slipping off her shoulders.

After applying 150 mg/kg NAC (n = 27, NAC group) bolus intravenously for 5 min, it was continued with 12.5 mg/kg/hour intravenously for 6 hours as an infusion.

In mERlow cells, both the 20 min and continued 60 min dephosphorylation were abrogated only with the PP2B inhibitor (Fig. 10b and 10c).

The gradient started with 15% B to 50% B from 0 to 16 min and continued isocratically up to 30 min.

Collapsing sites of extraction with sites of construction, Neo-Mining proposes continued mining phased with the construction of a new city directly on the Qinglonghu mines themselves.