Sentence examples for min were treated from inspiring English sources

Mitochondria isolated by differential centrifugation at 10,000 x g for 10 min were treated with proteinase K (Sigma) at a final concentration of 50 µg/ml for 25 min at 0°C.

Incidences separated by <30 min were treated as a single event.

Children with convulsions lasting ≥5 min were treated with a single dose (0.3 mg kg−1) of MDZ (Dormicum®; 15 mg per 3 ml; F. Hoffmann La Roche Ltd, Basel, Switzerland), administered IV as a slow bolus over 1 2 min (to reduce the chances of respiratory depression), or as an IM injection into the anterior aspect of the thigh, or via the buccal cavity.

Considering the severe brain pathology associated with generalized convulsive SE, any seizure lasting for more than 5 min is treated as an emergency in clinics [ 6, 7].

Continuing and sustained deviation of the ICP > 20 mmHg for longer than 30 min was treated by single doses of barbiturate (2 5 mg/kg) and by infusion of mannitol (0.5 g/kg in 15 min).

The protocol stipulates universal administration of 25 g of dextrose at 0 and 60 min after IV insulin, and hourly monitoring of blood glucose at 0, 60, 120 and 180 min. All blood glucose levels <3.9 mmol/L (70 mg/dL) at 120 or 180 min are treated with additional dextrose.

Transient severe hypotension (BP was 57 78/42 52 mm Hg that lasted for 25 min) was treated with reducing the sufentanil Ce to 0.05 ng/ml, and administering boluses of 6% hydroxyethyl starch 130/0.4 (Voluven ® 6%, Hospira, Fresenius Kabi, Halden, Norway), as well as phenylephrine (300 µg) and ephedrine (10 mg).

After incubation of BODIPY-BBN 5 (10 nM, 30 min), cells were treated with blue whole cell stain (5 min) and washed with PBS.

After baseline collection (5 min), cells were treated with 200 µM H2O2 for 55 min. Next measurements were done at DIV5 (after cell incubation with H2O2 during 60 min at DIV1).

PMNLs (2×10 cells) were incubated in 6 ml HBSS/Hepes medium at 37°C with or without test compounds for 30 min and were treated with zymosan or bacteria for 15 60 min (see figure legends).

After rinsing in PBS three times at room temperature for 5 min, sections were treated with proteinase K at 37 °C for 6 min.