In the index of the respective phase rotation vector, u min is sent to a receiver as side information (SI), comprising log2U bits.
Two weeks after visiting the website, an e-mail with a link to an online questionnaire (QuestionPro, mean duration to complete: 7.9 ± 6.5 min) was sent to invite employees who requested the advice to participate in the acceptability testing.
Three 1322T and three Min tumour samples were sent to the Paterson Institute Microarray Service where they were hybridised to GeneChip Mouse Exon 1.0 ST Arrays (Affymetrix, Santa Clara, CA, USA).
The time intervals were of 3 to 4 min and the light discharges were sent in cycles of 60 min from a distance of 3 4 m in an atmospheric environment and a low temperature of 4°C.
The following thermocycling conditions were used: 96 °C for 2 min, 35 cycles at 94 °C for 1 min, 48 °C for 1 min, 72 °C for 1 min, and a final step at 72 °C for 5 min. The PCR fragments were sent for sequencing at the University of Washington High Throughput Genomics Center for both the forward and reverse strands.
Baseline and test breath samples were collected in duplicate at time 0 and after 30 min. Tubes with breath samples were sent to a single analytical centre by post and measured within a 1-week period.
The experimental mouse was placed in the center chamber that was open to both sides and allowed to explore for 5 min. Video signals from digital cameras were sent to a desktop PC and processed online with Actimetrics LIMELIGHT software.
The samples were sent to the laboratory, and the serum and plasma were isolated by centrifugation (10 min, 3000 g).
The FMOC derivates were separated using the following linear gradient: 30-50% B in 15 min, 50-100% B in 20 min, 5 min isocratic elution at 100% B. The LC flow rate was set at 0.8 mL/min and after split 50 μL/min were sent to the mass spectrometer.
Four reminders were sent.
Blood cultures were sent.
