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Concentration of camphor and CCS was selected as independent variables whereas percentage porosity and percentage drug release at 60 min were selected as dependent variables.
Amount of drug released in 0.1 N hydrochloric acid at 2 h and in pH 6.8 phosphate buffer at 45 min were selected as responses.
The highest temperature assayed (75 °C), a moderate solvent concentration (50%) and an extraction time of 55 min were selected as the optimum extraction conditions using the response surface methodology.
Liposome vesicle size, drug entrapment efficiency and % drug release after 60 min were selected as CQAs and mathematical relationship between CQAs and CMAs was derived using multiple linear regression analysis.
TP optimal extraction conditions (53.15 mg GAE/g) were achieved after PLE (2 cycles, 80 °C/5 min,) while 1 cycle with 100 °C/15 min were selected as the optimal PLE conditions for TF recovery (16.51 mg QE/g).
Three factors including initial concentration of As III) (20 to 580 mg kg− 1 of soil), amount of added Fe (0.5 to 2.5 wt.% of soil for both Fe(II) and ZVI, 0.05 to 0.2 wt.% of soil for loaded Fe on zeolite) and shaking time (15 to 960 min) were selected as the independent factors on arsenic immobilization efficiency.
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Based on the results of the first step, n-hexane was chosen as desorption solvent and 5 min was selected as desorption time.
A robust and fast separation for the eight compounds within less than 6 min was selected and confirmed through experimental robustness testing.
Therefore, a contact time of 120 min was selected for the entire equilibrium test.
Thus 15 min was selected as optimum soaking time for the study.
Therefore, in subsequent experiments, 90 min was selected for contact times.
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