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A washout period of 60 min was maintained before the neurophysiological measurements were started.
Between any titre dosage, a hold time of 3 min was maintained.
Based on these results, a contact time of 30 min was maintained for all subsequent batch experiments.
The methanol gradient then increased to 50% over 5 min, increased to 90% over the next 30 min, was maintained at 90% for 10 min, and, finally, decreased to 30% over 1 min.
The gradient started at 10% B rose to 100% B in 6 min, was maintained at 100% B for 2 min and returned to 10% B for 2 min to initialize the system.
In electrophysiological experiments, a minimal interval of 2.5 min was maintained between stimulation pulses.
When fructose was given in combination with leptin, a rapid and significant increase in fructose-induced hyperglycaemia occurred as soon as 15 min. The +20% increase (P<0.05 vs. fructose) reached at 15 min was maintained over the 120 min period.
We analysed VV intervals ranging from −40 ms (LV pacing first) to +40 ms (RV pacing first), with steps of 20 ms. An equilibrium period of 1 2 min was maintained between echocardiographic recordings to allow haemodynamic stabilization.
The separation module, a Waters Alliance 2965 was operated in gradient mode at a flow rate of 300 μl min−1 for 30 min as follows: 0 2 min 95 5 (A B) followed by an increase in B from 5 to 95 in the following 26 min (2.01-28.00 min), thereafter returning to initial conditions (29.01-30.00 min) that were maintained for 5 min for column reconditioning.
The steady-state gradients were established within 1 min and were maintained for several minutes.
The mechanical ventilation continued for 15 min and rats were maintained in the reverse-Trendelenburg position.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com