Sentence examples for min were incubated from inspiring English sources

MDA-MB-231 cells were plated at 30,000 cells per well in Image Lock 96 well plates (Essen BioScience) in 100 μL complete DMEM medium and after allowing cells to settle for 20 min were incubated overnight at 37°C in 5% CO2 in a humidified incubator.

Cells washed twice with PBS (5 min) were incubated in 0.5 mL solution of 0.5 mg/mL RN-ase, at 37°C for 30 min.

In order to understand these interactions, two complexes: 1 (t1/2 < 1 min) and a previously studied 17 [Pt 5,6-dimethyl-1,10-phenanthroline)(1S,2S-diaminocyclohexane)] (56MESS) (GSH t1/2 = 4080 min) were incubated with rabbit plasma.

3 mg of sample, including a control sample boiled for 20 min, were incubated with a mixture containing 2.5 nmol/ml of sphingomyelin (Indofine) and 3pCi of [N-methyl-14C]Sphingomyelin (Amersham) in 45 ml total volume.

To verify that the band shifts were comprised of NF-κB, lysates from cells that were stimulated by TNF-α (20 ng/mL, 15 min) were incubated with anti-NF-κB p65 antibody (100 ng/mL, Zymed, S. San Francisco, CA).

Precleared cell-free supernatants (100,000 g, 30 min) were incubated at 4°C overnight with protein G beads (30 μl, Amersham Biosciences, Freiburg, Germany) loaded with 1 μg of the EpEX-specific antibody HO.3 [ 20].

BMP7 with or without heating at 80 °C for 2 min was incubated in the presence or absence of BMP7 antibodies (1 100 dilution) in cultured cells.

Bafilomycin A1 (5 μM; 30 min) was incubated with cells to block V-ATPase (vacuolar-type proton ATPase).

After three washes in TBS for 10 min, sections were incubated for 45 min with the secondary antibody at room temperature.

After incubation at 30°C for 60 min, samples were incubated at 97°C for 7 min to stop the reaction.

After rinsing with phosphate-buffered saline (PBS) for 15 min, sections were incubated with secondary antibody for 20 min and washed again with PBS for 10 min.