Sentence examples for min wash step from inspiring English sources

After the final 3 × 10 min wash step with TBST the membrane was submitted to AP-detection.

(ii) In case of β-glucosidase-activity, samples were prepared according to (i) and separated on a SDS-PAGE followed by a 1 min washing step in A. dest, 60 min washing in 1% (v/v) Triton X-100 and another step for 1 min in A. dest.

The separation of the two sample groups with one 3 min washing step using 4 M urea hydrogen peroxide was superior to three 5 min washing steps with 5 M urea (Table 1, Figure 1).

5 μm thick sections were deparaffinized and rehydrated through a descending alcohol series followed by a 4 min washing step in destilled H2O.

After a 3 × 5 min washing step with 1 × PBS, incubation with the second antibody coupled to Alexa488, Alexa568 or Alexa647 for 1 h followed.

Slides were washed twice with PBST and detection of serum auto-antibodies was performed by incubation with Cy3 conjugated Affini Pure rabbit anti human IgA + IgG + IgM (Jackson Immuno Research) in a dilution of 1 25000 in PBST plus 3% nonfat dried bovine milk for 2 h followed by a 5 min washing step in PBST.

Slides were blocked with DIGeasy Hyb (Roche Diagnostics, Mannheim, Germany) for 30 min, followed by two 5 min washing steps with PBST.

The blots were then subjected to three 5 min washing steps in TBST, after which they were incubated with the corresponding HRP-conjugated secondary antibodies: goat anti-rabbit (IgG), goat anti-mouse (IgG) or rabbit anti-goat (IgG) (1 10,000; Millipore Bioscience Research Reagents) for 1 hr at room temperature followed by three 10 min washing steps with TBST.

Reduce the remaining aldehydes with 3 successive 10 min washing steps with RB Rinse with 2 successive washing steps (200 µl each) in 10 mM TBS-Mg buffer for 10 min each.

To remove unbound antibodies, three 30-min washing steps with PBS were performed.

After three 5-min washing steps with PBST the animals were incubated with 1× Proteinase K in PBST for 7 min. The reaction was stopped by adding 4 mg/ml glycine in PBST.